Human Macrophage Activation Factors - I. Multiple Molecular Species of MAP Produced by a Human Lymphoid Cell Line
Document Type
Article
Publication Date
3-1-1980
Description
The lymphokine, macrophage activation factor (MAP), is defined functionally as the factor that activates mouse macrophages to kill syngeneic tumor cells. The source of MAP in this study is the Namalwa cell line, a human Burkitt's lymphoma cell line, that produces MAP as a constitutive product. The initial procedure in MAP characterization has been chromatography on Sepharose 6B-C1 column with the denaturating solvent, 6 M guanidine HC1, pH 8.0, in order to reduce the copurification of MAP activity with serum proteins. Namalwa cells incubated in RPMI 1640 with 2% fetal calf serum (FCS) produce MAF in the molecular weight ranges of 70,000 ± 7000, 25,000 ± 2500, 12,500 ± 1250 and less than 10,000; while Namalwa cells, incubated in 10% FCS, produce activity at 12,500 daltons and below. The MAF active fraction from 10% PCS supernatant was reincubated in 10% PCS for 24 hr, and on rechromatography the MAF activity appears from 70,000 to 12,500. Reincubation in 6 M guanidine moves the MAF activity to the column void volume (greater than 100,000 daltons) and to 70,000 daltons. The increase in molecular size of MAF suggests that MAF consists of a small basic molecular unit (less than 10,000 daltons) which associates in larger molecular forms upon incubation at 37° C.
Citation Information
McDaniel, Mildred C.. 1980. Human Macrophage Activation Factors - I. Multiple Molecular Species of MAP Produced by a Human Lymphoid Cell Line. Inflammation. Vol.4(1). 125-135. https://doi.org/10.1007/BF00914109 PMID: 7390612 ISSN: 0360-3997