A Fluorescent Water Soluable Carbodiimide-2-Hydroxy-3-Naphthoic Acid Hydrozide Reaction for the Demonstration of Carboxyl Groups in Proteins and Mucosubstances
Document Type
Article
Publication Date
6-1-1975
Description
The carbodiimide-2-hydroxy-3-naphthoic acid hydrazide reaction as developed by Geyer (1964) was used without subsequent diazonium coupling as a fluorescent method for the demonstration of carboxyl groups in both proteins and mucosubstances. The topological distribution of the fluorophore was similar to that reported by Geyer. Quantitative microfluorometric studies on cartilage sections revealed differences in detail between emissions in cartilage matrix mucoprotein as compared to the dense connective tissue associated with the perichondrium which consists principally of protein. It would also appear that the primary fluorescent emission of unstained preparations at 450 mm should be useful in microfluorometric determinations of proteins.
Citation Information
Curtis, Sherill K.; and Cowden, Ronald R.. 1975. A Fluorescent Water Soluable Carbodiimide-2-Hydroxy-3-Naphthoic Acid Hydrozide Reaction for the Demonstration of Carboxyl Groups in Proteins and Mucosubstances. Histochemistry. Vol.45(2). 115-120. https://doi.org/10.1007/BF00495155 PMID: 1194087 ISSN: 0301-5564