A Comparison of Two Methods of Preparing Cells or Nuclei for High-Resolution Microspectrophotometry
Document Type
Article
Publication Date
1-1-1976
Description
Suspensions of isolated mouse thymus cells were subjected to two preparative methods: either they were dropped through several mm of 9:1 v/v ethanol-acetic acid fixative, allowed to stand for 1 hr and then processed for staining; or they were fixed, passed through a graded ethanol series to 70% ethanol, centrifuged on to slides in a modified Shandon cytocentrifuge and then carried wet into the staining procedure. All preparations were stained by the Feulgen reaction and evaluated by high-resolution microspectrophotometry. While the two preparative procedures yielded similar results, there appeared to be less variability in the data obtained from the centrifuged cell populations.
Citation Information
Cowden, Ronald R.; and Curtis, Sherill K.. 1976. A Comparison of Two Methods of Preparing Cells or Nuclei for High-Resolution Microspectrophotometry. The Histochemical Journal. Vol.8(1). 77-85. https://doi.org/10.1007/BF01004008 PMID: 57951 ISSN: 0018-2214