Cytotoxicity to Allogeneic Cells in the Chicken. II. Specific Cytotoxic T Cells and Macrophages in the Spleens of Agammaglobulinemic and Normal Alloimmune Chickens
Document Type
Article
Publication Date
11-1-1981
Description
Repeated intravenous injections with allogeneic RBC or spleen cells caused a rapid but brief appearance of specific cellular cytotoxic activity in both NL and Aγ chickens. The activity was much higher in Aγ than in NL chickens, of longer duration, and contained a significant cytotoxic T-cell component which could not be demonstrated in NL chickens. A large fraction of the cytotoxic T cells adhered to Vicia villosa and showed relatively low density on Percoll gradients. Specificity of the cytotoxic cells in Aγ alloimmune chickens appeared to be directed at antigens of the major histocompatibility locus. Plastic-adherent cells from Aγ animals showed some specific and nonspecific killer activity. The specific component was removed by treatment with rabbit anti-T-cell serum and C. Specific cytotoxic activity in Aγ spleen cells was approximately equally distributed over Fc+ and Fc- cells. No evidence for specific cytotoxicity of nonT cells was obtained with cells from Aγ alloimmune chickens.
Citation Information
Chi, David S.; Blyznak, Nestor; Kimura, Arthur; Palladino, Michael A.; and Thorbecke, G. Jeanette. 1981. Cytotoxicity to Allogeneic Cells in the Chicken. II. Specific Cytotoxic T Cells and Macrophages in the Spleens of Agammaglobulinemic and Normal Alloimmune Chickens. Cellular Immunology. Vol.64(2). 246-257. https://doi.org/10.1016/0008-8749(81)90477-9 PMID: 6975662 ISSN: 0008-8749