Double-Label Analyses of the Coexistence of Somatostatin With GABA and Glycine in Amacrine Cells of the Larval Tiger Salamander Retina
Document Type
Article
Publication Date
7-16-1993
Description
To investigate the possible GABAergic nature of somatostatin-immunoreactive neurons of the larval tiger salamander retina, somatostatin immunocytochemistry was combined with either γ-aminobutyric acid (GABA) immunocytochemistry or autoradiography of GABA high-affinity uptake. A total of 1,062 somatostatin cells were visualized in these studies. Double-label immunocytochemistry revealed that 96.3% of somatostatin-immunoreactive cells expressed GABA immunoreactivity. Double-label studies combining somatostatin immunocytochemistry with autoradiography of GABA high-affinity uptake revealed a slightly lower percentage (93%) of colocalization. Double-labelled cells were identified as Type 1, Type 2 and displaced amacrine cells. The small percentage of somatostatin-immunoreactive cells that did not co-label for GABA were identified as Type 1 amacrine cells. An analysis of retinal sections processed for double-label immunocytochemistry revealed that approximately 5% of GABA-immunoreactive cells in the amacrine and ganglion cell layers co-label for somatostatin. Somatostatin immunocytochemistry was combined with autoradiography of glycine high-affinity uptake to examine whether tiger salamander somatostatin-amacrine cells express this glycine marker. A total of 100 somatostatin-immunoreactive amacrine cells were visualized in double-label preparations. None of these cells were observed to exhibit glycine high-affinity uptake.
Citation Information
Watt, Carl B.; and Florack, Valarie J.. 1993. Double-Label Analyses of the Coexistence of Somatostatin With GABA and Glycine in Amacrine Cells of the Larval Tiger Salamander Retina. Brain Research. Vol.617(1). 131-137. https://doi.org/10.1016/0006-8993(93)90623-U PMID: 8104080 ISSN: 0006-8993