Characterize the role of tobacco deacetylase enzyme SIP-428 in mediating environmental stress

Location

D.P. Culp Center Room 303

Start Date

4-5-2024 2:30 PM

End Date

4-5-2024 3:30 PM

Name of Project's Faculty Sponsor

Dhirendra Kumar

Faculty Sponsor's Department

Biological Sciences

Competition Type

Competitive

Type

Oral Presentation

Presentation Category

Science, Technology and Engineering

Abstract or Artist's Statement

Global climate change poses a significant threat to natural ecosystems and agriculture by reducing crop productivity and food security. Research projects focusing on abiotic stress tolerance in plants are crucial for addressing these challenges, promoting sustainable agricultural practices, and advancing our understanding of plant biology. Some plants have defense mechanisms that are activated upon receiving stress stimuli to increase systemic tolerance to abiotic stresses such as heat, light, and cold. Salicylic acid-binding protein 2 (SABP2) from tobacco exhibits a high affinity for salicylic acid (SA) and is an important component in the SA-signaling pathway. SABP2 interacts with other cellular proteins to initiate downstream signaling and activate responses leading to resistance. Several SABP2-interacting proteins (SIP), including SIP-428, have been identified. The main goal of this proposed research is to determine the role of SIP-428 in mediating environmental stresses. SIP-428 is a SIR2-type non-histone deacetylase enzyme. De/acetylation is a common post-translational modification of proteins in eukaryotes. Since SIP-428 is a SABP2-interacting protein, it is involved in plant immune signaling. To determine the role of SIP-428 in plant physiologytransgenic tobacco that overexpress SIP-428 (T3 generation) had been used in this study. These lines expressed SIP428 at higher levels upon treatment with β-estradiol. To test the role of overexpressed SIP428 in abiotic stress, the transgenic plants were treated with abiotic stress-inducing chemicals, e.g. NaCl (salinity stress), and mannitol (osmotic stress). The treated seedlings were allowed to grow for a specific time (1-2 weeks). The expression of SIP-428 was monitored by western blotting (using anti-myc as primary and HRPO-labelled anti-mouse secondary antibodies). The effects of SIP-428 expression on abiotic stress tolerance was investigated biochemically by examining the activities of antioxidant enzymes, catalase (CAT) and (POD) peroxidase. In this study, overexpressing SIP-428 in tobacco plants leads to diminished growth under stress from NaCl and mannitol, indicating a negative impact on stress tolerance. Overexpression of SIP428 specifically decreased the activity of catalase (CAT) but not peroxidase (POD), suggesting the negative regulatory role of SIP-428 in regulating CAT activity during abiotic stress. The imbalance in antioxidant enzyme activities due to overexpression of SIP428 may lead to the susceptibility of plants to abiotic stresses.

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Apr 5th, 2:30 PM Apr 5th, 3:30 PM

Characterize the role of tobacco deacetylase enzyme SIP-428 in mediating environmental stress

D.P. Culp Center Room 303

Global climate change poses a significant threat to natural ecosystems and agriculture by reducing crop productivity and food security. Research projects focusing on abiotic stress tolerance in plants are crucial for addressing these challenges, promoting sustainable agricultural practices, and advancing our understanding of plant biology. Some plants have defense mechanisms that are activated upon receiving stress stimuli to increase systemic tolerance to abiotic stresses such as heat, light, and cold. Salicylic acid-binding protein 2 (SABP2) from tobacco exhibits a high affinity for salicylic acid (SA) and is an important component in the SA-signaling pathway. SABP2 interacts with other cellular proteins to initiate downstream signaling and activate responses leading to resistance. Several SABP2-interacting proteins (SIP), including SIP-428, have been identified. The main goal of this proposed research is to determine the role of SIP-428 in mediating environmental stresses. SIP-428 is a SIR2-type non-histone deacetylase enzyme. De/acetylation is a common post-translational modification of proteins in eukaryotes. Since SIP-428 is a SABP2-interacting protein, it is involved in plant immune signaling. To determine the role of SIP-428 in plant physiologytransgenic tobacco that overexpress SIP-428 (T3 generation) had been used in this study. These lines expressed SIP428 at higher levels upon treatment with β-estradiol. To test the role of overexpressed SIP428 in abiotic stress, the transgenic plants were treated with abiotic stress-inducing chemicals, e.g. NaCl (salinity stress), and mannitol (osmotic stress). The treated seedlings were allowed to grow for a specific time (1-2 weeks). The expression of SIP-428 was monitored by western blotting (using anti-myc as primary and HRPO-labelled anti-mouse secondary antibodies). The effects of SIP-428 expression on abiotic stress tolerance was investigated biochemically by examining the activities of antioxidant enzymes, catalase (CAT) and (POD) peroxidase. In this study, overexpressing SIP-428 in tobacco plants leads to diminished growth under stress from NaCl and mannitol, indicating a negative impact on stress tolerance. Overexpression of SIP428 specifically decreased the activity of catalase (CAT) but not peroxidase (POD), suggesting the negative regulatory role of SIP-428 in regulating CAT activity during abiotic stress. The imbalance in antioxidant enzyme activities due to overexpression of SIP428 may lead to the susceptibility of plants to abiotic stresses.