Honors Program
University Honors
Date of Award
5-2018
Thesis Professor(s)
Dr. Ranjan Chakraborty
Thesis Professor Department
Health Sciences
Thesis Reader(s)
Dr. Sean Fox, Dr. Hugh Miller
Abstract
Iron is an essential nutrient for the proper functioning of many bacterial physiological processes. A deficiency of iron in bacteria can cause inhibition of cell growth and changes in morphology. Under iron restricted environments, microorganisms seek out different methods of acquiring iron. Our lab reported that RhizobiumLeguminosarum ATCC 14479, a gram-negative soil bacterium, is capable of utilizing heme as the sole source of iron. This work focuses on identifying and sequencing the hmuPSTUVoperon to assess its’ possible role in heme utilization in R. leguminosarum biovar trifoliiATCC 14479. We have confirmed the presence of this operon and have completed the sequencing of each gene involved in the operon. We have used in-silico analysis to assess the possible function of each gene. In the future, gene knockout will be performed to confirm the function of the hmuPSTUVoperon.
Publisher
East Tennessee State University
Document Type
Honors Thesis - Withheld
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Recommended Citation
Zimmer, Sarah Kathryn, "Identification of the Hmu PSTUV operon and its’ role in Heme Utilization in Rhizobia Leguminosarum ATCC 14479" (2018). Undergraduate Honors Theses. Paper 501. https://dc.etsu.edu/honors/501
Copyright
Copyright by the authors.
Included in
Biochemistry Commons, Environmental Microbiology and Microbial Ecology Commons, Microbial Physiology Commons, Molecular Biology Commons