Document Type
Article
Publication Date
7-23-2021
Description
We developed a spatially-tracked single neuron transcriptomics map of an intrinsic cardiac ganglion, the right atrial ganglionic plexus (RAGP) that is a critical mediator of sinoatrial node (SAN) activity. This 3D representation of RAGP used neuronal tracing to extensively map the spatial distribution of the subset of neurons that project to the SAN. RNA-seq of laser capture microdissected neurons revealed a distinct composition of RAGP neurons compared to the central nervous system and a surprising finding that cholinergic and catecholaminergic markers are coexpressed, suggesting multipotential phenotypes that can drive neuroplasticity within RAGP. High-throughput qPCR of hundreds of laser capture microdissected single neurons confirmed these findings and revealed a high dimensionality of neuromodulatory factors that contribute to dynamic control of the heart. Neuropeptide-receptor coexpression analysis revealed a combinatorial paracrine neuromodulatory network within RAGP informing follow-on studies on the vagal control of RAGP to regulate cardiac function in health and disease.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Citation Information
Moss, Alison; Robbins, Shaina; Achanta, Sirisha; Kuttippurathu, Lakshmi; Turick, Scott; Nieves, Sean; Hanna, Peter; Smith, Elizabeth H.; Hoover, Donald B.; Chen, Jin; Cheng, Zixi J.; Ardell, Jeffrey L.; Shivkumar, Kalyanam; Schwaber, James S.; and Vadigepalli, Rajanikanth. 2021. A Single Cell Transcriptomics Map of Paracrine Networks in the Intrinsic Cardiac Nervous System. iScience. Vol.24(7). https://doi.org/10.1016/j.isci.2021.102713
Copyright Statement
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).