Panning T Cells on Vascular Endothelial Cell Monolayers: A Rapid Method for Enriching Naive T Cells
Document Type
Article
Publication Date
1-1-2001
Description
A key functional/phenotypic difference between naive and memory T cells is the ability of memory and activated T cells to home to sites of inflammation by adhering to vascular endothelial cells. To determine if this trait could be used to separate naive T cells from memory T cells. CD4+ T cells were incubated with monolayers of IFN-γ-primed vascular endothelial cells after which the phenotypic and functional characteristics of the nonadherent population were assayed. The nonadherent population 1) contained a five-fold decrease in the frequency of cells displaying the CD44high/CD45RBlow "memory" phenotype and 2) responded well to allostimulation but displayed a reduced ability to respond to immobilized anti-CD3 antibody and, when isolated from ovalbumin-immunized mice, displayed a reduced recall response to ovalbumin in vitro. These studies demonstrate that two brief incubations of T cells with monolayers of IFN-γ-primed endothelial cells can significantly enrich for naive T cells as determined by both phenotypic and functional analyses.
Citation Information
Hoellman, John R.; Suttles, Jill; and Stout, Robert D.. 2001. Panning T Cells on Vascular Endothelial Cell Monolayers: A Rapid Method for Enriching Naive T Cells. Immunobiology. Vol.203(5). 769-777. https://doi.org/10.1016/S0171-2985(01)80005-X PMID: 11563676 ISSN: 0171-2985