Regulation of Eosinophil-Active Cytokine Production From Human Cord Blood-Derived Mast Cells
Document Type
Article
Publication Date
1-1-2002
Description
Human mast cells are multifunctional tissue-dwelling cells that play a crucial role in eosinophil-dependent disorders, such as asthma and parasitic diseases, by the secretion of eosinophil-active mediators. Mast cell-derived cytokines, generated in response to cross-linking of the high-affinity IgE receptor, can regulate eosinophil activation, survival, and chemotaxis. In this study, mast cells generated from human cord blood progenitors (stem cells) were studied for eosinophil-active inflammatory cytokine expression. Cord blood-derived mast cells (CBDMC) expressed typical intracellular scroll granules and microvilli-like structures on their cell surfaces, demonstrated the presence of tryptase, and elaborated prostaglandin D2 (PGD2) after cross-linkage of the high-affinity receptor for IgE (FcεRI). CBDMC expressed tumor necrosis factor-α (TNF-α) and the eosinophil-active growth factors, interleukin-5 (IL-5) and granulocyte-macrophage colony-stimulating factor (GM-CSF) after activation. (IL-1β greatly enhanced IgE-dependent production of these cytokines in response to FcεRI cross-linkage, suggesting a role for bystander/phagocytic cells in modulating mast cell function. In contrast, interferon-α (IFN-α) inhibited IL-5 and GM-CSF generation, and the glucocorticoid, dexamethasone (Dex), inhibited production of IL-5 and GM-CSF from CBDMC. A macrophage-mast cell-eosinophil axis may existin vivo that may be susceptible to pharmacologic manipulation.
Citation Information
Krishnaswamy, Guha; Hall, Kenton; Youngberg, George; Hossler, Fred; Johnson, David; Block, William A.; Huang, Shau Ku; Kelley, Jim; and Chi, David S.. 2002. Regulation of Eosinophil-Active Cytokine Production From Human Cord Blood-Derived Mast Cells. Journal of Interferon and Cytokine Research. Vol.22(3). 379-388. https://doi.org/10.1089/107999002753675811 PMID: 12034046 ISSN: 1079-9907