Endomorphin-2 Is Not Released From Rat Spinal Dorsal Horn in Response to Intraplantar Formalin
Document Type
Article
Publication Date
12-6-2002
Description
Antibody coated microprobes, inserted into the spinal cord at the L4-5 level, were used to detect whether endomorphin-2 (Endo2) was released from spinal dorsal horns in anesthetized rats in response to formalin injected into the hindpaw footpads. Saline injections were used as a control and substance P (SP) was measured to verify activation of nociceptive afferent fibers. SP but not Endo2 was released during pre-stimulation periods. Saline injections did not cause the release of either Endo2 or SP from the spinal cord. Formalin injections caused an increase in Fos expression as well as a release of SP, but not Endo2 from the ipsilateral side dorsal horn in L4-5. We conclude that Endo2 does not play a role in mediating the in vivo responses to acute inflammatory nociceptive signals at the spinal level in the anesthetized rat model.
Citation Information
Williams, Carole A.; Ricketts, Brian A.; Hua, Fang; and Dun, Nae J.. 2002. Endomorphin-2 Is Not Released From Rat Spinal Dorsal Horn in Response to Intraplantar Formalin. Neuroscience Letters. Vol.334(1). 53-57. https://doi.org/10.1016/S0304-3940(02)00998-9 PMID: 12431774 ISSN: 0304-3940