Ultra Low Concentrations of Morphine Increase Neurite Outgrowth in Cultured Rat Spinal Cord and Cerebral Cortical Neurons
Document Type
Article
Publication Date
7-15-2004
Description
The present study was undertaken to evaluate the effects of ultra low concentrations (10-9 or 10-14 M) of morphine on neurite elongation in cultured neurons dissociated from rat spinal cords and cerebral cortex. In fetal serum (FS) or fetal serum-free supplemented with cAMP media, the length of longest neurite was significantly increased by 10-9 or 10-14 M morphine. For example, 10-14 M morphine increased neurite length by 24±0.5% and 27±0.3% in spinal cord neurons, and 18±0.2% and 17±0.6% in cortical neurons. Morphine (10-6 M) had no significant effect on neurite length of spinal and cortical neurons. The relative frequency distribution of neurite length revealed 61±2.7% of spinal neurons and 48±2.6% of cortical neurons are responsive to ultra low concentrations of morphine. In the responsive populations, morphine (10 -14 M) enhanced the neurite outgrowth in spinal neurons by 58±0.9% and 48±1.2% and in cortical neurons by 31±0.6% and 28±0.9% in FS and cAMP-supplemented media, respectively. Pretreatment with naloxone did not prevent the morphine effect. The result shows that morphine at ultra low concentrations enhances neurite outgrowth of spinal and cortical neurons via a naloxone-independent mechanism.
Citation Information
Brailoiu, Eugen; Hoard, Jennifer; Brailoiu, G. Cristina; Chi, Michelle; Godbolde, Ramona; and Dun, Nae J.. 2004. Ultra Low Concentrations of Morphine Increase Neurite Outgrowth in Cultured Rat Spinal Cord and Cerebral Cortical Neurons. Neuroscience Letters. Vol.365(1). 10-13. https://doi.org/10.1016/j.neulet.2004.03.092 PMID: 15234463 ISSN: 0304-3940