Characterization of Estrogen-Responsive Epithelial Cell Lines and Their Infectivity by Genital Chlamydia Trachomatis
Document Type
Article
Publication Date
12-1-2005
Description
Chlamydial attachment and infectivity in vitro and ascending disease and sequelae in vivo have been reported to be enhanced/modulated by estrogen. Endometrial carcinoma cell lines Ishikawa and HEC-1B and the breast cancer lines MCF-7 and HCC-1806 were examined for Chlamydia trachomatis E infectivity. Estrogen receptor (ER) presence was confirmed by Western blot and qRT-PCR analyses. FACS analysis was used to determine the percent of plasma membrane-localized ERs (mERs), and their activity was tested by estrogen binding and competitive estrogen antagonists assays. Chlamydiae grew in all cell lines with HEC (90%) ≫ MCF-7 (57%) > Ishikawa (51%) ≫ HCC-1806 (20%). The cell line ER isoform composition was re-defined as: ERα + ERβ + for MCF-7, HCC-1806 and Ishikawa; and ERβ only for HEC-1B. HeLa cells were also tested and found to express ERβ, but not ERα. A small percentage of both ERs were surface-exposed and functionally active. The endometrium- predominant ERβ isoform was found in all cell lines, including those most representative of the common sites of C. trachomatis infection. Thus, the role of chlamydial attachment/infectivity will now be analyzed in ERβ + and - isogenic HEC-1B cells.
Citation Information
Guseva, Natalia V.; Dessus-Babus, Sophie C.; Whittimore, Judy D.; Moore, Cheryl G.; and Wyrick, Priscilla B.. 2005. Characterization of Estrogen-Responsive Epithelial Cell Lines and Their Infectivity by Genital Chlamydia Trachomatis. Microbes and Infection. Vol.7(15). 1469-1481. https://doi.org/10.1016/j.micinf.2005.05.004 PMID: 16046168 ISSN: 1286-4579