Blocking the MyD88-Dependent Pathway Protects the Myocardium From Ischemia/Reperfusion Injury in Rat Hearts
Document Type
Article
Publication Date
12-16-2005
Description
We examined whether blocking the MyD88 mediated pathway could protect myocardium from ischemia/reperfusion (I/R) injury by transfecting Ad5-dnMyD88 into the myocardium of rats (n = 8) 3 days before the hearts were subjected to ischemia (45 min) and reperfusion (4 h). Ad5-GFP served as control (n = 8). One group of rats was (n = 8) subjected to I/R without transfection. Transfection of Ad5-dnMyD88 significantly reduced infarct size by 53.6% compared with the I/R group (15.1 ± 3.02 vs 32.5 ± 2.59) while transfection of Ad5-GFP did not affect I/R induced myocardial injury (35.4 ± 2.59 vs 32.5 ± 2.59). Transfection of Ad5-dnMyD88 significantly inhibited I/R-enhanced NFκB activity by 50% and increased the levels of phospho-Akt by 35.6% and BCL-2 by 81%, respectively. Cardiac myocyte apoptosis after I/R was significantly reduced by 59% in the Ad5-dnMyD88 group. The results demonstrate that both inhibition of the NFκB activation pathway and activation of the Akt signaling pathway may be responsible for the protective effect of transfection of dominant negative MyD88.
Citation Information
Hua, Fang; Ha, Tuanzhu; Ma, Jing; Gao, Xiang; Kelley, Jim; Williams, David L.; Browder, I. William; Kao, Race L.; and Li, Chuanfu. 2005. Blocking the MyD88-Dependent Pathway Protects the Myocardium From Ischemia/Reperfusion Injury in Rat Hearts. Biochemical and Biophysical Research Communications. Vol.338(2). 1118-1125. https://doi.org/10.1016/j.bbrc.2005.10.068 PMID: 16259957 ISSN: 0006-291X