Safrole Oxide Inhibits Angiogenesis by Inducing Apoptosis
Document Type
Article
Publication Date
6-1-2005
Description
Our previous studies indicate that 3, 4-(methylenedioxy)-1-(2′, 3′-epoxypropyl)-benzene (safrole oxide), a newly synthesized compound, induces apoptosis in vascular endothelial cells (VECs) and A549 lung cancer cells. To our knowledge, the inhibition of angiogenesis by safrole oxide has not been reported yet. We report here that cultured rat aorta treated with safrole oxide exhibited a significant microvessel reduction as determined by counting the number of microvessels in a phase contrast microscope. There were more microvessels formed in the presence of A549 lung cancer cells in rat aorta model, while a dramatic inhibition of angiogenesis was obtained by adding 220-450 μmol l- 1 of safrole oxide to the growth medium (P <. 01). The culture of rat aorta treated with safrole oxide produced only some abortive endothelial cells but not microvessels. Furthermore, safrole oxide induced antiangiogenic effect in the chorioallantoic membranes (CAM) as a dose dependent manner. Eggs treated with 2-11 μmol 100 μl- 1 per egg of the safrole oxide for 48 h exhibited a significant reduction in blood vessel area of the CAM, a process likely mediated by apoptosis as demonstrated by DNA fragmentation. Our results suggest that safrole oxide has antiangiogenic activity and this effect might occur by induction of cellular apoptosis.
Citation Information
Zhao, Jing; Miao, Junying; Zhao, Baoxiang; Zhang, Shangli; and Yin, Deling. 2005. Safrole Oxide Inhibits Angiogenesis by Inducing Apoptosis. Vascular Pharmacology. Vol.43(1). 69-74. https://doi.org/10.1016/j.vph.2005.04.004 PMID: 15936989 ISSN: 1537-1891