Measurement of Mast Cell Cytokine Release by Multiplex Assay
Document Type
Article
Publication Date
1-1-2006
Description
Mast cells are highly responsive cells that are capable of secreting a variety of inflammatory mediators, including histamine, heparin, serine proteases, leukotrienes, prostaglandins, and thromboxanes. Studies from several laboratories have demonstrated that mast cells have the capacity to produce a variety of cytokines in response to various stimuli. Characterization of the cytokine profiles in mast cells has routinely been determined by the performance of individual enzyme-linked immunosorbent assays. This process is expensive, time-consuming, and requires a great deal of material to characterize multiple cytokines. In this chapter, we describe a multiplex cytokine assay to detect 17 cytokines simultaneously in 50 microL of culture supernatant derived from stimulated human cord blood-derived mast cells.
Citation Information
Breuel, Kevin; and De Ponti, W. Keith. 2006. Measurement of Mast Cell Cytokine Release by Multiplex Assay. Methods in molecular biology (Clifton, N.J.). Vol.315 217-230. https://link.springer.com/protocol/10.1385%2F1-59259-967-2%3A217 PMID: 16110161 ISSN: 1064-3745
