Mast Cell Histamine and Cytokine Assays
Document Type
Article
Publication Date
1-1-2006
Description
Mast cells are crucial to the development of chronic allergic inflammation and are likely to play a critical role in host defense. In this chapter methodology for histamine and cytokine assays is provided. Crosslinkage of IgE receptor I (Fc epsilonRI) on cord blood-derived mast cells by myeloma IgE and anti-human IgE is used to induce histamine release. Histamine levels were measured in the culture supernatants using an enzyme-linked immunosorbent assay. A human mast cell line (HMC-1), derived from a patient with mast cell leukemia, was activated with interleukin (IL)-1beta to study cytokine production and gene expression. Cytokine gene expression was evaluated by reverse transcriptase polymerase chain reaction and cytokine production was assayed in culture supernatants using an enzyme-linked immunosorbent assay kit.
Citation Information
Chi, David; Fitzgerald, S. Matthew; and Krishnaswamy, Guha. 2006. Mast Cell Histamine and Cytokine Assays. Methods in molecular biology (Clifton, N.J.). Vol.315 203-215. https://link.springer.com/protocol/10.1385%2F1-59259-967-2%3A203 PMID: 16110160 ISSN: 1064-3745