Assays for Nitric Oxide Expression
Document Type
Article
Publication Date
1-1-2006
Description
The purpose of this chapter is to review the analytical methodology specifically associated with studying the role of nitric oxide (NO) in mast cell physiology and biochemistry. The methodology for measuring cellular secretion of nitric oxide with Griess Reagent will be described in detail, as well as the use of 4,5-diaminofluorescein diacetate for continuous monitoring of nitric oxide production in live cells. We will point out the limitations of the analytical techniques and also indicate areas in which promising analytical techniques have not yet been applied to the study of mast cell physiology, that is, new research opportunities. In addition to reviewing the methodology associated with measuring NO itself, we will briefly touch upon some analytical methods important in characterizing the biochemical products formed from nitric oxide (e.g., 3-nitrotyrosine).
Citation Information
Stone, William; Yang, Hongsong; and Qui, Min. 2006. Assays for Nitric Oxide Expression. Methods in molecular biology (Clifton, N.J.). Vol.315 245-256. https://link.springer.com/protocol/10.1385%2F1-59259-967-2%3A245 PMID: 16110163 ISSN: 1064-3745