Soluble Dectin-1 as a Tool to Detect β-Glucans

Creator(s)

Lisa Graham, University of Cape Town, Faculty of Health Sciences
S. Vicky Tsoni, University of Cape Town, Faculty of Health Sciences
Janet A. Willment, University of Cape Town, Faculty of Health Sciences
David L. Williams, Quillen-Dishner College of MedicineFollow
Philip R. Taylor, Sir William Dunn School of Pathology
Siamon Gordon, Sir William Dunn School of Pathology
Kevin Dennehy, University of Cape Town, Faculty of Health Sciences
Gordon D. Brown, University of Cape Town, Faculty of Health Sciences

Document Type

Article

Publication Date

7-31-2006

Description

β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

Citation Information

Graham, Lisa; Tsoni, S. Vicky; Willment, Janet A.; Williams, David L.; Taylor, Philip R.; Gordon, Siamon; Dennehy, Kevin; and Brown, Gordon D.. 2006. Soluble Dectin-1 as a Tool to Detect β-Glucans. Journal of Immunological Methods. Vol.314(1-2). 164-169. https://doi.org/10.1016/j.jim.2006.05.013 PMID: 16844139 ISSN: 0022-1759