Identification of a Peptide Sequence That Improves Transport of Macromolecules Across the Intestinal Mucosal Barrier Targeting Goblet Cells
Document Type
Article
Publication Date
6-1-2008
Description
In this study, we demonstrated that the CSKSSDYQC-peptide ligand which was identified from a random phage-peptide library through an in vivo phage display technique with rats could prominently improve the transport efficiency of macromolecules, such as large filamentous phage particles (M13 bacteriophage), across the intestinal mucosal barrier. Synthetic CSKSSDYQC-peptide ligands significantly inhibited the binding of phage P1 encoding CSKSSDYQC-peptide ligands to the intestinal mucosal tissue and immunohistochemical analysis showed that the CSKSSDYQC-peptide ligands could be transported across the intestinal mucosal barrier via goblet cells as their specific gateway. Thus, we inferred that CSKSSDYQC-peptide ligand might have a specific receptor on the goblet cells and transported from intestinal lumen to systemic circulation by transcytosis mechanism. These results suggest that CSKSSDYQC-ligand could be a promising tool for development of an efficient oral delivery system for macromolecular therapeutics in the carrier-drug conjugate strategy.
Citation Information
Kang, Sang; Woo, Jung Hee; Kim, Min Kook; Woo, Sang Soo; Choi, Jin Hyuk; Lee, Hong Gu; Lee, Nam Kyung; and Choi, Yun Jaie. 2008. Identification of a Peptide Sequence That Improves Transport of Macromolecules Across the Intestinal Mucosal Barrier Targeting Goblet Cells. Journal of Biotechnology. Vol.135(2). 210-216. https://doi.org/10.1016/j.jbiotec.2008.01.021 PMID: 18440083 ISSN: 0168-1656