HGF/SF and Menthol Increase Human Glioblastoma Cell Calcium and Migration
Document Type
Article
Publication Date
7-18-2008
Description
This study explored the role of transient receptor potential melastatin 8 ion channels (TRPM8) in mechanisms of human glioblastoma (DBTRG) cell migration. Menthol stimulated influx of Ca2+, membrane current, and migration of DBTRG cells. Effects on Ca2+ and migration were enhanced by pre-treatment with hepatocyte growth factor/scatter factor (HGF/SF). Effects on Ca2+ also were greater in migrating cells compared with non-migrating cells. 2-Aminoethoxydiphenyl borate (2-APB) inhibited all menthol stimulations. RT-PCR and immunoblot analysis showed that DBTRG cells expressed both mRNA and protein for TRPM8 ion channels. Two proteins were evident: one (130-140 kDa) in a plasma membrane-enriched fraction, and a variant (95-100 kDa) in microsome- and plasma membrane-enriched fractions. Thus, TRPM8 plays a role in mechanisms that increase [Ca2+]i needed for DBTRG cell migration.
Citation Information
Wondergem, Robert; Ecay, Tom W.; Mahieu, Frank; Owsianik, Grzegorz; and Nilius, Bernd. 2008. HGF/SF and Menthol Increase Human Glioblastoma Cell Calcium and Migration. Biochemical and Biophysical Research Communications. Vol.372(1). 210-215. https://doi.org/10.1016/j.bbrc.2008.05.032 PMID: 18485891 ISSN: 0006-291X