A Polypeptide From Chlamys Farreri Inhibits UVB-Induced Hacat Cells Apoptosis via the Apaf-1/Caspase-9 and Smac/Xiap Signaling Pathway
Document Type
Article
Publication Date
9-1-2009
Description
A novel marine active polypeptide (PCF), isolated from the gonochoric Chinese scallop, Chlamys farreri, has potential antioxidant and anti-apoptotic activity against ultraviolet irradiation. We investigated whether UVB-induced HaCaT cell apoptosis occurs via the mitochondrial pathways Apaf-1/caspase-9 and Smac/XIAP/caspase-3. We then investigated the molecular mechanisms controlling the anti-apoptotic effect of PCF. Pre-treatment with PCF and caspase-9 inhibitor significantly inhibited UVB-induced apoptosis in HaCaT cells based on a DNA fragmentation assay and Hoechst 33258 staining. The expression of Apaf-1 and the cleavage of procaspase-9 were dose-dependently reduced by 1.42-5.96 mmol/L PCF pretreatment in UVB-irradiated HaCaT cells. This was followed by inhibition of cleavage of procaspase-3, whose activation induced cell apoptosis. Meanwhile, PCF significantly and dose-dependently enhanced the activation of ATPase. Furthermore, we demonstrated that PCF strongly inhibited the release of Smac from the mitochondria to cytosol by reducing the degradation of XIAP dose-dependently. We conclude that the protective effect of PCF against UVB irradiation in HaCaT cells may be attributed to the inhibition of the Apaf-1/caspase-9 and Smac/XIAP/caspase-3 apoptotic signaling pathways.
Citation Information
Liu, Xiaojin; Wang, Wencheng; Wang, Hongjiang; Zhang, Lanlan; Liu, Leqian; Wang, Yuejun; and Wang, Chunbo. 2009. A Polypeptide From Chlamys Farreri Inhibits UVB-Induced Hacat Cells Apoptosis via the Apaf-1/Caspase-9 and Smac/Xiap Signaling Pathway. Chinese Journal of Oceanology and Limnology. Vol.27(3). 587-593. https://doi.org/10.1007/s00343-009-9179-y ISSN: 0254-4059