Document Type
Article
Publication Date
1-1-2014
Description
Rebamipide is a mucoprotective agent commonly used to prevent nonsteriodal anti-inflammatory drug-induced gastrointenstinal side effects [1]. Human plasma and urine analysis of rebamipide utilizing high performance liquid chromatography (HPLC) have been reported [2]. Recently, we reported on the plasma levels of rebamipide in presense or absence of celecoxib or diclofenac in rats [3] using a modified HPLC method of detection developed by Jeoung et al. [4]. To tailor the method towards use in urinary rebamipide extraction and analysis, the following modifications were made:To compensate for high concentrations of rebamipide found in urine, a new rebamipide stock solution was prepared with a final concentration of 50,000 ng/mL.Rat urine calibration standards were obtained within the range of 50-1000 ng/mL and 1000-50,000 ng/mL.Plasma samples were replaced with urine samples.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Citation Information
Cooper, Dustin L.; and Harirforoosh, Sam. 2014. A Simple High Performance Liquid Chromatography Method for Determination of Rebamipide in Rat Urine. MethodsX. Vol.1(1). 49-55. https://doi.org/10.1016/j.mex.2014.06.002
Copyright Statement
ß 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/3.0/).