Hydrogen Peroxide Released From Pyropia yezoensis Induced by Oligo-Porphyrans: Mechanisms and Effect
Document Type
Article
Publication Date
1-1-2015
Description
In this study, oligo-porphyrans, obtained by acid hydrolysis of porphyran, were investigated for their H2O2-inducing abilities in the defense responses of P. yezoensis. Oligo-porphyrans with average molecular weights (MWs) lower than 1.43 kDa had H2O2-inducing abilities. In contrast, oligo-porphyrans with average MWs of 6.12 kDa triggered no response. The active oligo-porphyrans were fractioned by anion-exchange chromatography. We found that two distinct mechanisms might be involved in the oligo-porphyran-induced H2O2 release in P. yezoensis. Mixtures of mono-sulfated oligo-galactans with degrees of polymerization (DPs) ranging from 1 to 3 might induce the response through the oxidation of cellular oligosaccharides, which enable P. yezoensis to resist rotting caused by dense incubation. Mixtures of oligo-porphyrans, consisting of 4 ~ 7 monosaccharide residues and 2 ~ 3 sulfate groups, might induce the generation of H2O2 by activation of NADPH oxidase, leading to an oxidative burst in P. yezoensis. The elicitor activity of oligo-porphyrans thus depends on their molecular size.
Citation Information
Hou, Yun; Wang, Jing; Simerly, Thomas; Jin, Weihua; Zhang, Hong; and Zhang, Quanbin. 2015. Hydrogen Peroxide Released From Pyropia yezoensis Induced by Oligo-Porphyrans: Mechanisms and Effect. Journal of Applied Phycology. Vol.27(4). 1639-1649. https://doi.org/10.1007/s10811-014-0471-7 ISSN: 0921-8971