Document Type
Article
Publication Date
5-21-2015
Description
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. We examined the thymoquinone induced inhibition of purified F1 or membrane bound F1FO E. coli ATP synthase. Both purified F1 and membrane bound F1FO were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in both membrane bound F1Fo and purified F1 preparations. Moreover, thymoquinone induced inhibition of ATP synthase expressing wild-type E. coli cell growth and non-inhibition of ATPase gene deleted null control cells demonstrates that ATP synthase is a molecular target for thymoquinone. This also links the beneficial dietary based antimicrobial and anticancer effects of thymoquinone to its inhibitory action on ATP synthase.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Citation Information
Ahmad, Zulfiqar; Laughlin, Thomas F.; and Kady, Ismail O.. 2015. Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth. PLoS ONE. Vol.10(5). https://doi.org/10.1371/journal.pone.0127802 PMID: 25996607
Copyright Statement
© 2015 Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited