A cDNA Encoding the Highly Immunodominant Antigen of Strongyloides stercoralis: γ-Subunit of Isocitrate Dehydrogenase (NAD+)
Document Type
Article
Publication Date
1-1-2000
Description
A full length cDNA encoding the highly immunodominant 41 kDa antigen of Strongyloides stercoralis (P5), recognized by 83% of human patients [Siddiqui et al. (1997) Parasitol Res 83:655-658], is obtained. A clone containing a 1371 bp insert was selected following screening of the S. stercoralis cDNA library with antibodies specific to antigen P5. The nucleotide sequence of this insert identified a cDNA coding for the γ-subunit of isocitrate dehydrogenase (NAD+), GenBank Accession Number AF176568. The conceptually translated amino acid sequence of the open reading frame for the γ-subunit of S. stercoralis isocitrate dehydrogenase (NAD+) encodes a 388 amino acid residue protein with an apparent molecular weight of 43 kDa and a predicted pI of 7.15. The sequence is 71% A/T, reflecting the characteristic A/T codon bias of S. stercoralis. The amino acid sequence of the S. stercoralis γ-subunit of isocitrate dehydrogenase (NAD+) is compared with those of Caenorhabditis elegans, rat and human NAD+-ICDH. The diagnostic potential of the S. stercoralis γ-subunit of isocitrate dehydrogenase (NAD+) is also discussed.
Citation Information
Siddiqui, Afzal A.; Stanley, Christy S.; and Berk, Steven L.. 2000. A cDNA Encoding the Highly Immunodominant Antigen of Strongyloides stercoralis: γ-Subunit of Isocitrate Dehydrogenase (NAD+). Parasitology Research. Vol.86(4). 279-283. https://doi.org/10.1007/s004360050043 PMID: 10780735 ISSN: 0932-0113