Mutants of Listeriolysin O for Enhanced Liposomal Delivery of Macromolecules
Document Type
Article
Publication Date
4-5-2013
Description
Delivery of macromolecules into the cytosolic space of eukaryotic cells is a pressing challenge in biopharmaceutics. Macromolecules are often encapsulated into liposomes for protection and improved distribution, but the their size often induces endocytosis of the vehicle at the target site, leading to degradation of the cargo. Listeriolysin O is a key virulence factor of Listeria monocytogenes that forms pores in the endosomal membrane, ultimately allowing the bacterium to escape into the cytosol. This function of LLO has been used to improve cytosolic delivery of liposomally encapsulated macromolecules in a number of instances, but its innate toxicity and immunogenicity have prevented it from achieving widespread acceptance. Through site-directed mutagenesis, this study establishes a mutant of LLO (C484S) with enhanced activity, allowing for a reduction in the amount of LLO used for future applications in liposomal drug delivery.
Citation Information
Walls, Zachary F.; Goodell, Stefanie; Andrews, Chasity D.; Mathis, Jonathan; and Lee, Kyung Dall. 2013. Mutants of Listeriolysin O for Enhanced Liposomal Delivery of Macromolecules. Journal of Biotechnology. Vol.164(4). 500-502. https://doi.org/10.1016/j.jbiotec.2013.01.029 PMID: 23416330 ISSN: 0168-1656