Expression of the Cyclin-Dependent Kinase Inhibitor p27Kip1 by Developing Retinal Pigment Epithelium
Document Type
Article
Publication Date
10-1-2003
Description
The cyclin-dependent kinase (Cdk) inhibitor p27Kip1 contributes to the timing of cell cycle withdrawal during development and, consequently, in organogenesis. Within the retina, this effector protein is up-regulated during the birth of neuronal and glial cells [Dev. Biol. (2000) 299]. However, its expression within the retinal pigment epithelium (RPE), a supporting cell layer that is essential for neural retina development and function, has not previously been reported. We show that p27Kip1 protein expression in the RPE occurs in two phases: an up-regulation during mid-to late embryonic stages and a down-regulation during the subsequent postnatal period. In the early phase of up-regulation, an inverse relationship is seen between expression of p27Kip1 and PCNA, an indicator of cycling cells. During both up-and down-regulation, the change in spatial pattern of expression proceeds in a central to peripheral manner, with p27Kip1 up-regulation paralleling retinal maturation. These data suggest that this cell cycle regulator may be an important factor controlling the timing of RPE cell cycle withdrawal.
Citation Information
Defoe, Dennis M.; and Levine, Edward M.. 2003. Expression of the Cyclin-Dependent Kinase Inhibitor p27Kip1 by Developing Retinal Pigment Epithelium. Gene Expression Patterns. Vol.3(5). 615-619. https://doi.org/10.1016/S1567-133X(03)00120-0 PMID: 12971995 ISSN: 1567-133X