Oxidative Damage to Cytosine: Implication for the Study of Radiation-Induced Damage to DNA

Document Type

Article

Publication Date

1-23-2003

Description

A radical observed in irradiated single crystals of cytidine, 3′-CMP, and 5′-dCMP is characterized by three anisotropic proton hyperfine couplings and is called the 3αH radical. The 3αH radical shares properties with the allyl-like radical observed in thymine derivatives. The goal of the present work is to show that the previously observed 3αH radicals are cytosine base radicals formed on 5-methylcytosine impurities in these crystals. Ab initio electron propagator calculations in the partial third-order (P3) approximation with the 6-311G(d,p) basis set have been used to show that these 5-methylcytosines are good hole traps, having an ionization potential comparable to that of guanine. The importance of these cytosine oxidation sites to the radiation chemistry of DNA is discussed.

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