Modulation of Spontaneous Transmitter Release From the Frog Neuromuscular Junction by Interacting Intracellular CA2+ Stores: Critical Role for Nicotinic Acid-Adenine Dinucleotide Phosphate (Naadp)
Document Type
Article
Publication Date
7-15-2003
Description
Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a recently described potent intracellular Ca2+-mobilizing messenger active in a wide range of diverse cell types. In the present study, we have investigated the interaction of NAADP with other Ca2+-mobilizing messengers in the release of transmitter at the frog neuromuscular junction. We show, for the first time, that NAADP enhances neurosecretion in response to inositol 1,4,5-trisphosphate (IP3), cADP-ribose (cADPR) and sphingosine 1-phosphate (S1P), but not sphingosylphosphorylcholine. Thapsigargin was without effect on transmitter release in response to NAADP, but blocked the responses to subsequent application of IP3, cADPR and S1P and their potentiation by NAADP. Asynchronous neurotransmitter release may therefore involve functional coupling of endoplasmic reticulum Ca2+ stores with distinct Ca2+ stores targeted by NAADP.
Citation Information
Brailoiu, Eugen; Patel, Sandip; and Dun, Nae J.. 2003. Modulation of Spontaneous Transmitter Release From the Frog Neuromuscular Junction by Interacting Intracellular CA2+ Stores: Critical Role for Nicotinic Acid-Adenine Dinucleotide Phosphate (Naadp). Biochemical Journal. Vol.373(2). 313-318. https://doi.org/10.1042/BJ20030472 PMID: 12749764 ISSN: 0264-6021