Modulation of TRPV4 Gating by Intra- and Extracellular Ca²⁺

Creator(s)

Hiroyuki Watanabe, KU Leuven
Joris Vriens, KU Leuven
Annelies Janssens, KU Leuven
Robert Wondergem, East Tennessee State University
Guy Droogmans, KU Leuven
Bernd Nilius, KU Leuven

Document Type

Article

Publication Date

1-1-2003

Description

We have studied the modulation of gating properties of the Ca2+-permeable, cation channel TRPV4 transiently expressed in HEK293 cells. The phorbol ester 4αPDD transiently activated a current through TRPV4 in the presence of extracellular Ca2+. Increasing the concentration of extracellular Ca2+ ([Ca2+]e) reduced the current amplitude and accelerated its decay. This decay was dramatically delayed in the absence of [Ca2+]e. It was also much slower in the presence of [Ca2+]e in a mutant channel, obtained by a point mutation in the 6th transmembrane domain, F707A. Mutant channels, containing a single mutation in the C-terminus of TRPV4 (E797 , were constitutively open. In conclusion, gating of the 4αPDD-activated TRPV4 channel depends on both extra- and intracellular Ca2+, and is modulated by mutations of single amino acid residues in the 6th transmembrane domain and the C-terminus of the TRPV4 protein.

Citation Information

Watanabe, Hiroyuki; Vriens, Joris; Janssens, Annelies; Wondergem, Robert; Droogmans, Guy; and Nilius, Bernd. 2003. Modulation of TRPV4 Gating by Intra- and Extracellular Ca²⁺. Cell Calcium. Vol.33(5-6). 489-495. https://doi.org/10.1016/S0143-4160(03)00064-2 PMID: 12765694 ISSN: 0143-4160