Electrophoretic Evidence of Esterase Inhibition in Larval Caddisflies Exposed to Inorganic Mercury
Document Type
Article
Publication Date
1-1-1997
Description
In an previous 72-hr mercury bioassay with the larval caddisfly Nectopsyche albida, electrophoretically detectable esterase activity was absent in exposed individuals that succumbed to mercury toxicity, while nine other enzymes remained active hours after death. Esterase activity also persisted in unexposed individuals (Benton and Guttman, 1992a.b). To test the effects of mercury exposure duration on esterase activity, additional larval N. albida were exposed under conditions identical to those in the earlier bioassay, and esterase activity in live individuals was tested electrophoretically every 12 hr. To test the effects of mercury concentration on esterase activity, unexposed N. albida larvae were electrophoresed, and the esterase-specific stain was spiked with various concentrations of mercury. Electrophoretic banding patterns were then densitometrically quantified to identity changes in esterase activity with exposure duration and mercury concentration. Results suggest that: inorganic mercury inhibited esterase activity in N. albida, inhibition increased with exposure duration, and inhibition increased with mercury concentration.
Citation Information
Benton, Michael J.; and Guttman, Sheldon I.. 1997. Electrophoretic Evidence of Esterase Inhibition in Larval Caddisflies Exposed to Inorganic Mercury. Water Environment Research. Vol.69(2). 240-243. https://doi.org/10.2175/106143097X125407 ISSN: 1061-4303