Nitric Oxide Production: A Mechanism of Chlamydia Trachomatis Inhibition in Interferon-γ-Treated RAW264.7 Cells
Document Type
Article
Publication Date
1-1-1996
Description
IFN-γ and/or LPS induced nitrite production and inhibition of Chlamynia trachomatis (CT) replication in the murine macrophage cell line, RAW264.7. Linear regression analysis demonstrated a strong correlation between nitrite production and inhibition of CT replication (correlation coefficients: -0.93, P < 0.001). L-NMMA specifically inhibited nitrite production and restored CT replication (55-71%). Inducible nitric oxide synthase (iNOS) mRNA was analyzed by Northern and dot blot hybridization with an iNOS cDNA probe. A strong correlation between iNOS mRNA expression and inhibition of CT replication also was observed (correlation coefficient: -0.97, P < 0.05). Furthermore, anti-TNF-α antibody, which completely neutralized biological activity of the secreted TNF-α neither inhibited nitrite production nor restored CT replication in the LPS- and/or IFN-γ-treated RAW264.7 cells. In mouse peritoneal macrophages treated with IFN-γ, both L-NMMA and anti-TNF-α antibody inhibited nitrite production and restored CT replication. However, L-NMMA and the antibody had no effect upon nitrite production and CT inhibition in LPS-treated peritoneal macrophages. These data indicate that NO production is one mechanism for inhibition of CT replication in IFN-γ-activated murine macrophages.
Citation Information
Chen, Bojun; Stout, Robert; and Campbell, William F.. 1996. Nitric Oxide Production: A Mechanism of Chlamydia Trachomatis Inhibition in Interferon-γ-Treated RAW264.7 Cells. FEMS Immunology and Medical Microbiology. Vol.14(2-3). 109-120. https://doi.org/10.1016/0928-8244(96)00018-1 PMID: 8809546 ISSN: 0928-8244