Identification of the α_1C-Adrenoceptor in Rabbit Arteries and the Human Saphenous Vein Using the Polymerase Chain Reaction

Creator(s)

Nicole L. Diehl, Quillen-Dishner College of Medicine
S. Martin Shreeve, Quillen-Dishner College of Medicine

Document Type

Article

Publication Date

8-16-1994

Description

The expression of the α1C-adrenoceptor subtype in human and rabbit blood vessels has been analyzed using the reverse transcriptase/polymerase chain reaction technique (RT/PCR). The 20 bp primers employed were designed from the bovine α1C-adrenoceptor and flank a least conserved region - the putative third cytoplasmic loop. RT/PCR products generated from rabbit and human brain mRNA both had 93% homology to the bovine α1C-adrenoceptor and were used as species and subtype specific probes in Southern blot analysis of vascular RT/PCR products. Poly A+ RNA was purified from the human saphenous vein and rabbit aorta, renal, pulmonary and central ear arteries and amplified by RT/PCR. Size analysis by agarose gel electrophoresis, together with Southern hybridization of the resulting cDNA products confirm the expression of the α1C-adrenoceptor in these vessels.

Citation Information

Diehl, Nicole L.; and Martin Shreeve, S.. 1994. Identification of the α_1C-Adrenoceptor in Rabbit Arteries and the Human Saphenous Vein Using the Polymerase Chain Reaction. European Journal of Pharmacology: Molecular Pharmacology. Vol.268(3). 393-398. https://doi.org/10.1016/0922-4106(94)90064-7 PMID: 7805763 ISSN: 0922-4106