Treatment of Sperm With High-Ionic Strength Medium Increases Microsurgical Fertilization Rates of Rabbit Oocytes Fertilized by Subzonal Placement of Sperm
Document Type
Article
Publication Date
4-1-1991
Description
This study was conducted to investigate the requirement for sperm processing in microsurgical subzonal placement of sperm in rabbit oocytes. Fertilization rates with standard in vitro fertilization and microsurgical subzonal sperm placement were found to be similar (56 and 55%) when sperm treated with high-ionic strength Brackett's defined inedium to initiate capacitation were used. Statistically significant reductions in fertilization rates for both standard in vitro fertilization and subzonal placement were noted when twice-washed spermatozoa were used. Initiation of capacitation of spermatozoa results in higher fertilization results even when the zona pellucida is bypassed during fertilization.
Citation Information
Minhas, Brijinder S.; Roudebush, William E.; Ricker, Deborah D.; and Dodson, Melvin G.. 1991. Treatment of Sperm With High-Ionic Strength Medium Increases Microsurgical Fertilization Rates of Rabbit Oocytes Fertilized by Subzonal Placement of Sperm. Journal of In Vitro Fertilization and Embryo Transfer. Vol.8(2). 111-115. https://doi.org/10.1007/BF01138664 PMID: 2061679 ISSN: 0740-7769