Plasma Levels and Kinetic Disposition of 2,4-Dinitrophenol and Its Metabolites 2-Amino-4-Nitrophenol and 4-Amino-2-Nitrophenol in the Mouse
Document Type
Article
Publication Date
1-1-1985
Description
Eleven groups of six ICR mice were dosed orally with 22.5 mg/kg 2,4-dinitrophenol. Groups were sacrificed at 0, 0.5, 1, 2, 4, 6, 9, 12, 24, 48, and 96 h post-treatment and plasma was collected for analysis of dinitrophenol, 2-amino-4-nitrophenol, and 4-amino-2-nitrophenol content. Analyses were performed by capillary gas spectrometry after liquid-liquid extraction of plasma specimens spiked with two internal standards. Quantification was based upon peak-area ratios of base peaks obtained from the three analytes and the trideuterated internal standards 2,4-dinitrophenol and 2-amino-4-nitrophenol. Plasma concentrations for each analyte versus thei subjected to pharmacokinetic analysis. Of the two monoamine metabolites, 2-amino-4-nitrophenol was present in the greater amount and had an elimination plasma while that of 4-amino-2-nitrophenol was 26 h.
Citation Information
Robert, T. A.; and Hagardorn, A. N.. 1985. Plasma Levels and Kinetic Disposition of 2,4-Dinitrophenol and Its Metabolites 2-Amino-4-Nitrophenol and 4-Amino-2-Nitrophenol in the Mouse. Journal of Chromatography B: Biomedical Sciences and Applications. Vol.344(C). 177-186. https://doi.org/10.1016/S0378-4347(00)82018-6 PMID: 4086539 ISSN: 0378-4347