A Domain That Assumes a Z-Conformation Includes a Specific Deletion in Some Cloned Variants of a Complex Satellite
Document Type
Article
Publication Date
11-15-1988
Description
Sequence analyses show that deletions of 10 and 12 bp occur at homologous sites in a domain that is rich in alternating purines and pyrimidines (Pu/Py) in B42 and EXT, two cloned variants of a complex satellite DNA. A 3-bp deletion occurs 27 bp upstream from the site of the specific deletions in B42 and RU, a third cloned satellite variant that has not suffered the 10-bp deletion. Under torsional stress, the Pu/Py-rich domain adopts a Z-conformation as shown by (i) inhibition of cutting at a BssHII site that accounts for built2 5 of a 15-bp tract of pure Pu/Py in the domain; (ii) binding of polyclonal and monoclonal anti-Z-DNA antibodies to the domain; and (iii) antibody stabilization and subsequent relaxation of the Z-region.
Citation Information
Fowler, Richard F.; Stringfellow, Leslie A.; and Skinner, Dorothy M.. 1988. A Domain That Assumes a Z-Conformation Includes a Specific Deletion in Some Cloned Variants of a Complex Satellite. Gene. Vol.71(1). 165-176. https://doi.org/10.1016/0378-1119(88)90088-1 PMID: 3215523 ISSN: 0378-1119