MIF-I and Postsynaptic Receptor Sites for Dopamine
Document Type
Article
Publication Date
1-1-1979
Description
In an attempt to determine the mechanism by which the tripeptide l-prolyl-l-leucyl-glycine amide (PLG, MIF-I) exerts its antiparkinsonian effect, the action of this substance on various postsynaptic components of striatal dopaminergic nerves was studied. It was shown that injection of rats with MIF-I (1 mg/kg, IP×5, 24 hr intervals) did not alter tyrosine hydroxylase, dopa decarboxylase, choline acetyltransferase and glutamic acid decarboxylase activities in the striatum under the conditions tested. The activities of adenylate cyclase, dopamine-stimulated adenylate cyclase, and guanylate cyclase were not altered in vitro by various concentrations of MIF-I (0.1 to 1000 μM), although VIP and neurotensin had some effect. Also the rate of uptake of 3H-dopamine by rat striatal synaptosomes was unchanged, as was the binding of 3H-dopamine and 3H-spiperone to beef caudate membranes. This series of studies indicates that MIF-I does not act directly on the striatal dopamine postsynaptic receptor under the conditions tested, although it is possible that MIF-I could act indirectly at this or another site in vivo by releasing or activating some other factor.
Citation Information
Kostrzewa, Richard M.; Hardin, Judy C.; Snell, Robert L.; Kastin, Abba J.; Coy, David H.; and Bymaster, Frank. 1979. MIF-I and Postsynaptic Receptor Sites for Dopamine. Brain Research Bulletin. Vol.4(5). 657-662. https://doi.org/10.1016/0361-9230(79)90109-6 PMID: 39665 ISSN: 0361-9230