Binding of [³H]Quinuclidinyl Benzilate to Regions of Rat Pituitary and Hypothalamus

Creator(s)

Donald B. Hoover, Quillen-Dishner College of MedicineFollow
John C. Hancock, Quillen-Dishner College of MedicineFollow
Nancy S. Talley, Quillen-Dishner College of Medicine

Document Type

Article

Publication Date

1-1-1981

Description

Muscarinic ligand binding sites in fragments of rat hypothalamus and pituitary were studied using [3H]quinuclidinyl benzilate (QNB). In the hypothalamus, the highest amount of specific QNB binding was to n. paraventricularis and n. dorsomedialis. Specific QNB binding in other hypothalamic regions varied within a relatively narrow range. Fragments of whole pituitary also bound QNB but to a much smaller degree than brain. Pituitary binding of QNB was blocked by atropine but not by hexamethonium or d-tubocurarine. Within the pituitary, specific QNB binding to posterior pituitary was three times greater than to anterior pituitary. These findings are consistent with the operation of cholinergic mechanisms in hypothalamic and pituitary function.

Citation Information

Hoover, Donald B.; Hancock, John C.; and Talley, Nancy S.. 1981. Binding of [³H]Quinuclidinyl Benzilate to Regions of Rat Pituitary and Hypothalamus. Brain Research Bulletin. Vol.6(3). 209-211. https://doi.org/10.1016/S0361-9230(81)80049-4 PMID: 7225899 ISSN: 0361-9230