Real-Time Detection of Mitochondrial Inhibition at Frog Motor Nerve Terminals Using Increases in the Spatial Variance in Probability of Transmitter Release
Document Type
Article
Publication Date
2-13-1995
Description
The effects of Hg2+, methyl mercury, and flufenamic acid, all of which inhibit mitochondria, were examined at frog motor nerve terminals. Unbiased estimates of m (no. of transmitter quanta released), n (no. of functional release sites), p (probability of release), and vars p (spatial variance in p) were obtained using K+-induced asynchronous neurosecretion (m, n and p not having the same definitions as with nerve-evoked release). Transient but significant increases in m, n, p and vars p were found with all three agents. These findings indicate that mitochondrial inhibition and release of sequestered Ca2+ can be detected as a real-time increase in vars p. The results also suggest that changes in vars p might be used to differentiate between cellular (membrane) and subcellular (organellar) actions of drugs at the nerve terminal.
Citation Information
Provan, Spencer D.; and Miyamoto, Michael D.. 1995. Real-Time Detection of Mitochondrial Inhibition at Frog Motor Nerve Terminals Using Increases in the Spatial Variance in Probability of Transmitter Release. Neuroscience Letters. Vol.185(3). 187-190. https://doi.org/10.1016/0304-3940(95)91365-O PMID: 7753488 ISSN: 0304-3940