Demonstration of Sulfhydryl and Disulfide Groups by a Fluorescent Maleimide Procedure
Document Type
Article
Publication Date
1-1-1980
Description
Several fluorescent maleimide compounds were evaluated as possible substitutes for N-(4-aminophenyl)maleimide in the histochemical procedures developed by Sippel (1973, 1978a, b, 1980) for the demonstration of sulfhydryl and disulfide groups. The brightest and most selective fluorescence was obtained by using N-(7-dimethylamino-4-methylcoumarinyl)maleimide (DACM), although both eosin-5-maleimide and fluorescein-5-maleimide could also be used if adequate control preparations were made.
Citation Information
Curtis, Sherill K.; and Cowden, Ronald R.. 1980. Demonstration of Sulfhydryl and Disulfide Groups by a Fluorescent Maleimide Procedure. Histochemistry. Vol.68(1). 23-28. https://doi.org/10.1007/BF00498497 PMID: 7190963 ISSN: 0301-5564