Microfluorometric Estimates of Proteins Associated With Murine Hepatocyte and Thymocyte Nuclei, Residual Structures, and Nuclear Matrix Derivatives
Document Type
Article
Publication Date
7-1-1985
Description
Isolated diploid hepatocyte and thymocyte nuclei and their derivatives ("residual structures" and nuclear matrices, as defined by Kaufmann et al. 1981) were evaluated by microfluorometry following reaction with the following fluorochromes: brilliant sulfaflavine (BSF) used at pH 2.8 for the demonstration of total protein; acridine orange (AO) used at pH 9.0 to reveal acidic groups of proteins; and 3-(4-maleimidylphenyl)-7-diethylamino-4-methylcoumarin (CPM) used under conditions required to demonstrate the sum of sulfhydryl (SH) and disulfide (SS) groups of proteins. The results suggested that the proteins reacting with AO and CPM differed from each other and from those revealed by fluorochroming with BSF. In every comparison, hepatocyte nuclei and their derivatives were more fluorescent than the respective populations of thymocyte nuclei and their derivatives. In material fluorochromed with BSF and AO, nuclear matrices were less fluorescent than residual structures, which, in turn, were less fluorescent than intact nuclei. In contrast, nuclear matrices fluorochromed with CPM were less fluorescent than intact nuclei but more fluorescent (paradoxically) than residual structures. The ratios of the total fluorescence values of hepatocyte and thymocyte nuclei fluorochromed with BSF changed significantly during extractions required to produce residual structures and nuclear matrices, while comparable ratios in material fluorochromed with AO or CPM did not change significantly. Comparisons of the ratios of the fluorescence values of intact nuclei and their derivatives in a variety of combinations yielded complex and variable results. In their totality, the results obtained in the present investigation suggested that microfluorometric data can provide a useful complement to data derived by biochemical and other approaches if sufficiently large numbers of preparations and end-group procedures are utilized.
Citation Information
Curtis, S. K.; and Cowden, R. R.. 1985. Microfluorometric Estimates of Proteins Associated With Murine Hepatocyte and Thymocyte Nuclei, Residual Structures, and Nuclear Matrix Derivatives. Histochemistry. Vol.82(4). 331-339. https://doi.org/10.1007/BF00494061 PMID: 4008276 ISSN: 0301-5564