Molecular Properties of the Vasoactive Intestinal Peptide Receptor in Aorta and Other Tissues
Document Type
Article
Publication Date
1-1-1992
Description
The molecular weight of the vasoactive intestinal peptide (VIP) receptor was assessed in bovine aorta, and rat liver, lung, and brain by covalent cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The receptor in all four tissues was found to be a single polypeptide of approximate Mr 54,000, contradicting previous claims for substantial heterogeneity in the molecular weight of this receptor. Guanine nucleotides inhibit cross-linking of 125I-VIP to its receptor, and cross-linking with ethylene glycolbis(succinimidylsuccinate) provides further evidence for complex formation between VIP, its receptor and a guanine nucleotide-binding regulatory protein (G-protein). The precise mechanism of receptor-G-protein coupling may differ between the aorta and other tissues.
Citation Information
Shreeve, S. M.; DeLuca, Alexander W.; Diehl, Nicole L.; and Kermode, John C.. 1992. Molecular Properties of the Vasoactive Intestinal Peptide Receptor in Aorta and Other Tissues. Peptides. Vol.13(5). 919-926. https://doi.org/10.1016/0196-9781(92)90050-D PMID: 1336189 ISSN: 0196-9781