Enhancement of in Vitro Antibody Responses by Human Serum Proteins Cleaved by Trypsin
Document Type
Article
Publication Date
1-1-1985
Description
Tryptic cleavage of human serum proteins remarkably enhanced in vitro plaque-forming cell (PFC) responses of mouse spleen cells primed with sheep erythrocytes (SE). Moreover, trypsin cleaved proteins could mimic the activity of T-cells in immune responses of SE-primed B cells. Eluted with 6 M guanidine-HCl on a Sepharose 6B-CL Column, the activity was found in the fraction corresponding to the molecular weight greater than 100,000. The results suggest that tryptic cleavage of human serum proteins produced a factor(s) that resembeled the T-cell augmentation of B-cell immune responses.
Citation Information
Chi, David S.; and McDaniel, Mildred C.. 1985. Enhancement of in Vitro Antibody Responses by Human Serum Proteins Cleaved by Trypsin. International Journal of Immunopharmacology. Vol.7(1). 81-85. https://doi.org/10.1016/0192-0561(85)90012-8 PMID: 3888869 ISSN: 0192-0561