Characterization of Mutants of Ceratopteris Richardii Selected on Aluminum (Al₂(so₄)₃-Na₂EDTA)

Creator(s)

S. R. Wright, The University of Tennessee, Knoxville
L. G. Hickok, The University of Tennessee, Knoxville
T. R. Warne, East Tennessee State University

Document Type

Article

Publication Date

1-1-1990

Description

Mutant strains of the fern Ceratopteris richardii Brongn. were selected in vitro for tolerance to culture medium containing Al2(SO4)3-Na2EDTA at pH 4.4. Three strains, HαAT3, HαAT7, and HαAT29 showed enhanced growth relative to the wild type on selection medium. Because of the complex nature of the selection conditions, the mutants were tested for tolerance to low pH and other individual components of the selection medium. All three mutant strains were notably more tolerant to acidic conditions and slightly more tolerant to Na2EDTA, relative to the wild type. No consistent differences in response to Na2SO4 were evident. Genetic characterization indicated a single nuclear gene basis of inheritance for strain HαAT3. Strains HαAT7 and HαAT29 showed a nuclear basis of tolerance, but responses of gametophytes from F1 hybrids suggested segregation at two or more loci.

Citation Information

Wright, S. R.; Hickok, L. G.; and Warne, T. R.. 1990. Characterization of Mutants of Ceratopteris Richardii Selected on Aluminum (Al₂(so₄)₃-Na₂EDTA). Plant Science. Vol.68(2). 257-262. https://doi.org/10.1016/0168-9452(90)90232-D ISSN: 0168-9452