A Rapid Method for the Purification of RNA Polymerase Holoenzyme From Escherichia Coli
Document Type
Article
Publication Date
1-1-1990
Description
A method is described for the rapid purification of RNA polymerase holoenzyme from small amounts of Escherichia coli cells. Chromatography of a crude extract on a single-stranded DNA agarose column followed by gell filtration chromatography gave 95% pure holoenzyme. The enzyme kinetic characteristics on T7 DNA identical to those of RNA polymerase purified by other more laborious procedures.
Citation Information
Mehrpouyan, Majid; and Champney, W. Scott. 1990. A Rapid Method for the Purification of RNA Polymerase Holoenzyme From Escherichia Coli. Journal of Biochemical and Biophysical Methods. Vol.21(4). 289-297. https://doi.org/10.1016/0165-022X(90)90004-V PMID: 2089071 ISSN: 0165-022X