Chemical Reactivity and DNA Sequence Specificity of Formally Monofunctional and Bifunctional Bis(Platinum) Complexes
Document Type
Article
Publication Date
5-15-1994
Description
The synthesis of the formally monofunctional bis(platinum) complex [{Pt(NH}3)3μ-H2N(CH2)4NH2-{trans-PtCl(NH3)2}]3+ (1,0/t) is reported. The interactions of this species and the formally bifunctional bis(platinum) complex [{trans-PtCl(NH3)2}2H2N(CH2) NH2]Cl2 (1,1/t,t) with DNA were investigated. Comparison was made with the monomeric [PtCl(dien)]Cl, (Pt(DIEN)), and cis-[PtCl2(NH3)2], (cis-DDP). The initial rates of reaction with small self-complementary oligonucleotides 5′-ATATATN4ATATAT-3′ (N4 = GCGC and N4 = GGCC) were calculated. For all compounds, the GGCC oligonucleotide reacted faster than the GCGC counterpart. The order of reactivity of the platinum compounds for the GCGC oligonucleotide was 1,1/t,t > 1,0/t > Pt(DIEN) > cis-DDP. The reaction of 1,0/t and 1,1/t,t with poly(dGdC)·poly(dGdC) was also investigated using circular dichroism (CD) spectroscopy where both compounds were shown to induce a B → Z conformational change.
Citation Information
Wu, Philip K.. 1994. Chemical Reactivity and DNA Sequence Specificity of Formally Monofunctional and Bifunctional Bis(Platinum) Complexes. Journal of Inorganic Biochemistry. Vol.54(3). 207-220. https://doi.org/10.1016/0162-0134(94)80014-6 PMID: 8027742 ISSN: 0162-0134