IgM Rheumatoid Factor Elaboration by Blood, Bone Marrow, and Synovial Mononuclear Cells in Patients With Rheumatoid Arthritis
Document Type
Article
Publication Date
1-1-1985
Description
IgM rheumatoid factor (RF) elaboration by rheumatoid arthritis (RA) synovial, bone marrow, and blood mononuclear cells (MNC) is reported. IgM RF was prepared from RF-positive sera by sequential euglobulin precipitation, Sephacryl S300 gel filtration, and IgG-Sepharose affinity chromatography. Purified material, which contained no detectable IgG or IgA, was used in an enzyme-linked immunosorbent assay (ELISA) to quantitate cellular elaboration of IgM RF. Excellent standard curves (r2 {reversed tilde equals} 0.98) were obtained without nonspecific binding of samples or antisera to IgG-coated microtiter plates and without cross-reactivity of standards with antisera other than anti-IgM. We found RA blood MNC (11 patients) spontaneously averaged 15 ng/ml IgM RF (6% of total IgM produced), but elaborated 254 ng/ml IgM RF following pokeweed mitogen (PWM) stimulation (22 patients), exceeding that of 13 normal controls. Bone marrow MNC spontaneously (4 patients) produced 71 ng/ml IgM RF and secreted 78 ng/ml IgM RF with PWM stimulation (9 patients). In contrast synovial fluid MNC (5 patients) spontaneously elaborated 6652 ng/ml IgM RF, significantly (P < 0.05) more than blood or bone marrow MNC; PWM-stimulated synovial fluid MNC (5 patients) produced 5472 ng/ml IgM RF. These observations confirm selective localization of activated, IgM RF-producing cells to the rheumatoid synovial space.
Citation Information
Panush, Richard S.; Bittner, Anna K.; Sullivan, Marie; Katz, Paul; and Longley, Selden. 1985. IgM Rheumatoid Factor Elaboration by Blood, Bone Marrow, and Synovial Mononuclear Cells in Patients With Rheumatoid Arthritis. Clinical Immunology and Immunopathology. Vol.34(3). 387-391. https://doi.org/10.1016/0090-1229(85)90187-4 PMID: 3871679 ISSN: 0090-1229