Effect of Temperature on Suppressor Cells in Chicken Spleen Cell Cultures

Document Type

Article

Publication Date

1-1-1984

Description

The plaque forming cell (PFC) response of in vivo primed chicken spleen cells to sheep erythrocytes (SRBC) at 37°C in vitro was much higher when antigen was added on Day 2 of culture than on Day 0, at the initiation of the incubation period. No such difference was observed when the cells were cultured at 40°C, in which case both responses were low. As shown previously the effect of delayed exposure to SRBC at 37°C was due to a disappearance of suppressor T cells in the absence of antigen, which did not occur at 40°C. Addition of concanavalin A on Day 0 could, even in the absence of SRBC, maintain the suppressor cell activity at 37°C. The results suggest that suppressor cell activity is very temperature dependent.

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