Immunocytochemical Evidence for Particulate Localization of Phenylethanolamine-N-Methyltransferase in Adrenal Medulla

Creator(s)

L. S. Van Orden, Quillen-Dishner College of Medicine
J. P. Burke, Quillen-Dishner College of Medicine
Jan A. Redick, Quillen-Dishner College of Medicine
K. E. Rybarczyk, Quillen-Dishner College of Medicine
Dianna E. Van Orden, Quillen-Dishner College of Medicine
Harriet A. Baker, Quillen-Dishner College of Medicine
B. K. Hartman, Quillen-Dishner College of Medicine

Document Type

Article

Publication Date

1-1-1977

Description

Previous biochemical evidence for the localization of phenylethanolamine-N-methyltransferase in adrenal medulla has indicated that this enzyme is soluble, although some workers have reported a significant amount of particulate phenylethanolamine-N-methyltransferase (Joh and Goldstein, 1973). Electron microscopic immunocytochemical study of bovine adrenal medulla has revealed a distinct particulate localization of phenylethanolamine-N-methyltransferase in association with chromaffin granules. It is therefore suggested that some, and possibly most, adrenal phenylethanolamine-N-methyltransferase may be in a particulate form in intact tissue, and that solubilization artifacts during tissue preparation could account in part for the large proportion of soluble phenylethanolarmine-N-methyltransferase reported previously.

Citation Information

Van Orden, L. S.; Burke, J. P.; Redick, Jan A.; Rybarczyk, K. E.; Van Orden, Dianna E.; Baker, Harriet A.; and Hartman, B. K.. 1977. Immunocytochemical Evidence for Particulate Localization of Phenylethanolamine-N-Methyltransferase in Adrenal Medulla. Neuropharmacology. Vol.16(2). 129-133. https://doi.org/10.1016/0028-3908(77)90060-0 PMID: 840371 ISSN: 0028-3908